Coding

Part:BBa_K764023:Design

Designed by: Frederic Dudbridge   Group: iGEM12_Exeter   (2012-09-16)

Cyclodextrin glycosyltransferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 471
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Synthesised with the correct biobrick primers


Source

This gene was synthesised by reverse translating the amino acid sequence by codon-optimisation, <Bacillus Subtilits</i> (Strain 1011), Accession Number: 1942571.


References

Harata, K. et al. (1996) X-ray structure of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. 1011. Comparison of two independent molecules at 1.8 A resolution, Acta. Crystallogr. D. Biol. Crystallogr. 52: 1136-1145.